While the free cortisol pattern in saliva has value and is considered the gold standard, there is a significant missing piece to surveying the HPA-Axis function with saliva testing – cortisol metabolites are not measured. To properly characterize cortisol status, free and metabolized cortisol should be measured to avoid misleading results when cortisol clearance is abnormally high or low. Likewise with sex hormones, measuring estrogen and androgen metabolites gives a bigger picture for more precise analysis of hormonal imbalances and HRT monitoring.
Here is a list of answers to the most common questions here.
Simply collect the sample as instructed the following day. All
samples do not need to be collected in one 24-hour period.