CERTIFIED NUTRITIONIST    •    
BOARD-CERTIFIED IN HOLISTIC NUTRITION® 
   •    CERTIFIED FUNCTIONAL MEDICINE PRACTITIONER

ION® Profile with 40 Amino Acids

About the ION Profile

The ION® (Individual Optimal Nutrition) Profile tests 150 markers with 40 Amino Acids nutritional analysis to help identify nutrient deficiencies that may be a root cause of complex chronic conditions. The ION with 40 Amino Acids is ideal for establishing a baseline assessment ahead of clinical intervention with nutraceuticals as well as subsequent monitoring as needed.

Who is a ION Profile for?

This test is one of the most comprehensive nutrient assessment tests available. Those who have symptoms of nutrient imbalances may benefit from this test. Symptoms such as:

  • Mood Disorders
  • Depression
  • Anxiety
  • Fatigue
  • Digestive Complaints
  • Chronic Pain/Inflammatory Conditions
  • Musculoskeletal
  • Migraine
  • Cardiovascular Risk
  • Weight Issues/Dietary Guidance
  • General Health and Sports Fitness Optimization

How much is an ION?

You can expect to pay approximately $445. (Please put your credit card number or check on the requisition. If the price is not on the requisition – please fill in this price). The price is subject to change

What are the advantages of an ION?

Using both blood and urine, the ION Profile with 40 Amino Acids is an advanced nutritional analysis that offers insight into imbalances that may precede abnormal findings on standard laboratory evaluations.

The complete ION Profile with 40 Amino Acids includes:

  • 40 Amino Acids
  • Nutrient and Toxic Elements
  • CoEnzyme Q10 plus Vitamins
  • DNA/Oxidative Stress Markers
  • Vitamin D
  • Fatty Acids
  • Organic Acids

IMPORTANT TEST PREPARATION

READ CAREFULLY: 

• It is best to ship your specimen within 24 hours of collection. Please refer to the enclosed shipping instructions before you collect to determine what days you can ship your specimen.

• It is not necessary to discontinue nutritional supplements prior to this specimen collection. Abnormalities that may be found will reveal special needs that have not been met by recent dietary and supplemental intake.

• Decrease fluid intake to avoid excessive dilution of the urine.
» For adults, restrict intake to three 8 oz. glasses or less for 24 hours.
» Make sure that no more than 8 oz. of fluids are consumed after 8:00 the evening prior to urine collection.

• Do Not collect urine during menstruation.

• Vial contains preservative - Do Not Rinse

• Schedule a morning blood drawing appointment on a Monday, Tuesday, Wednesday, or Thursday. Inform the doctor or lab that a centrifuge is needed to prepare the blood specimens. The kit contains all of the tubes required for collection.

• Do not have anything to eat or drink (other than water) after 9:00 on the night before your blood is drawn.

• Staple payment to the bottom right-hand corner of the completed Test Requisition Form and Complete the Personal Health Assessment Form; Fold and Place them in the side pocket of the biohazard bag. 

• Take frozen urine specimen (placed in biohazard bag with frozen ice packet) and ALL collection and shipping materials with you to the blood drawing site. This will allow the blood and urine specimens to be shipped together to the lab. 

Frequently Asked Questions Regarding ION Testing

You can download a copy of the instructions for clients HERE.

You can download a copy of clinician instructions HERE.

What are the collection Requirements
Have a clean container available to urinate into (a large plastic cup will work)
• It is best to ship your specimen within 24 hours of collection. Please refer to the
enclosed shipping instructions before you collect to determine what days you can
ship your specimen.

• It is not necessary to discontinue nutritional supplements prior to this specimen
collection. Abnormalities that may be found will reveal special needs that have
not been met by recent dietary and supplemental intake.
• Decrease fluid intake to avoid excessive dilution of the urine.
» For adults, restrict intake to three 8 oz. glasses or less for 24 hours.
» Make sure that no more than 8 oz. of fluids are consumed after 8:00 the evening
prior to urine collection.
• Do Not collect urine during menstruation.
• Vial contains preservative - Do Not Rinse.
How do I ship the sample?
Specimens may be shipped Monday through Thursday.

Before shipping, be sure that all the samples and the Test Request Form are labeled and completely filled out including payment.

All test tubes should be placed into the bubble bags (provided) and placed inside the test kit box after completing all required breath specimens. The box should be mailed back to the laboratory with the prepaid postage (included) within two days of completion of the test.  If you collect your urine at home you will need to ensure the facility who is drawing your blood has the urine sample to include it with your blood samples to get returned to the lab.


Locate the FedEx Clinical Pak mailer. Fill in your name and address on the shipping label attached to the outside of mailer. Place the kit box into FedEx Clinical Pak. Remove strip to reveal sticky film and press both sides of mailer together to seal the pouch.


Call FedEx to schedule a pick-up. Dial 1-800-238-5355. When the automated greeting begins say “Rep”. When asked if you are shipping a package say “Yes”. A live person will then answer to help schedule your pickup. Let them know you are shipping using a Billable Stamp or take to a FedEx shipping center.
Do I have to pay for postage?
Yes, please follow the packing and shipping instructions provided with the test kit.
Can I drop the kit off at a FedEx location?
Yes, please follow the packing and shipping instructions provided with the test kit.
I accidentally threw out the test kit box, can I use another one to ship?
Yes, use any clean small box.
I sealed the bag but forgot to put the paperwork in it can I reopen it?
Yes, just tape it reclosed.
Can I ship my sample on Friday?
No, ship specimens to the lab only Monday – Thursday. The box should be mailed back to the laboratory with the prepaid postage (included) within two days of completion of the test.
My test kit did not have a requisition form, what do I do?
Unless you already received the test requisition in your private portal, you should email or call our office (at info@fortitudefunctionalnutrition.com or 720-580-2078) for a Test Requisition Form.
695 S. Colorado Blvd
Suite 340
Denver, CO 80246

(720) 580-2078
© Copyright 2023 - Fortitude Functional Nutrition 
All Rights Reserved
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